![]() DNA–protein conformations) directly from tethered particle motion data with better resolution than existing methods, while easily correcting for common experimental artifacts. ![]() ![]() It learns the number of distinct states (i.e. Our method, vbTPM, performs variational Bayesian inference in hidden Markov models. To probe this issue, we have developed a new analysis method for tethered particle motion, a versatile and commonly used in vitro single-molecule technique. Despite being one of the best-studied model systems for transcriptional regulation, the number and conformations of loop structures accessible to LacI remain unclear, though the importance of multiple coexisting loops has been implicated in interactions between LacI and other cellular regulators of gene expression. The bacterial transcription factor LacI loops DNA by binding to two separate locations on the DNA simultaneously.
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